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KMID : 0357119930150010107
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Korean Journal of Immunology
1993 Volume.15 No. 1 p.107 ~ p.118
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Induction of Effector and Suppressor cells of Contact Hypersensitivity in Normal and UVR-exposed Mice
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Jun Byoung-Duek
Kang Kyoung-Jin
Chai Ok-Hee
Lee Moo-Sam
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Abstract
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Ultraviolet radiation (UVR0 caused suppression of cntact hyperdsensitivity (CH). Further, UVR-induced suppression of CH is mediated, in part, by antigen-specific suppressor T-cells(Ts cells). The purpose of this study was to characterize CH
effector and Ts cell development in the lymphoid organs of normal mice and determine how UVR affects this process. Normal and UVR exposed mice were sensitized with a high dose of 0.5% DNFB(25μl, on abdomen:10μl, on each ear and foot), 5 days total peripheral lymph nodes (LN) and spleen cells were transferred to syngeneic recipients. They were chanllenged with 0.1% DNFBS on the right ear and afger 24d hours the ear swelling response was measured. CH effector cells activity was observed in LN and spleen of both normal and UVR-exposed donors. When recipients of these cells were sensitized after an dadoptive transfer and challenged 5 edays later, Ts cell activity was observed in the LN and spleen of both normal and UVR-exposed donors. Using low dose 0.5% DNFB sensitizzat ion (25μl, abdomen only) effector cells were transferred with LN and spleen from normal and UVR-exposed donors, however, Ts cells activity was observed from the LN, and not from the spleen, of normal mice. Theses results inducated that normal immune responses to high dose DNFB induce both effector and Ts cell activities in Ts cell development only in the LN, and UVR shifts the dose response curve to DNFB such that low doses of DNFB induce Ts cells in spleen and LN.
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KEYWORD
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Ultraviolet radiation(UVR), Contact hypersensitivity(CH), Effector and suppressor T cells
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